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The Effects of Atrazine Exposure on DNA Methylation in Drosophila melanogaster

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dc.contributor.author Nicolodi, Victoria
dc.date.accessioned 2017-05-29T21:08:01Z
dc.date.available 2017-05-29T21:08:01Z
dc.date.issued 2017-05
dc.identifier.uri http://hdl.handle.net/11416/323
dc.description Honors Thesis Spring 2017 en_US
dc.description.abstract Atrazine is the second most commonly used herbicide in the United States with 80 million pounds being applied to farmlands yearly. It is a potent endocrine disrupter, as it acts as a xenoestrogen and causing estrogen dominance. Atrazine has been observed to cause behavioral, developmental, and reproductive changes in species such as frogs, salmon, and rats. Current research is looking at DNA methylation, which is the addition of a methyl group to the fifth carbon on the cytosine ring, as an epigenetic factor for gene expression controlling cellular processes such as gene suppression and genomic imprinting, In this study, Drosophila melanogaster were exposed to varying concentrations of atrazine and their DNA was extracted to observe and compare the changes in global methylation patterns. It is predicted that there were be global DNA hypomethylation in flies exposed to atrazine compared to those of the control. Data obtained showed that was some correlation between viable candidates produced and the concentration of the exposure environment, but not enough to be definitive. Through setbacks in the DNA extraction process, techniques for genomic DNA extraction from Drosophila melanogaster was developed. en_US
dc.publisher Florida Southern College en_US
dc.subject Atrazine en_US
dc.subject Herbicide en_US
dc.subject Endocrine disrupters en_US
dc.title The Effects of Atrazine Exposure on DNA Methylation in Drosophila melanogaster en_US
dc.type Thesis en_US

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